Effects of Eucalyptus wood and leaf litter on saproxylic insects in the southeastern United States

Although Eucalyptus is widely planted outside its native range for timber and pulp production, the effects of these exotic plantations on biodiversity relative to native semi-natural forests or plantations of native tree species remain incompletely understood. Here, we compare the diversity of saproxylic beetles (Coleoptera) and true bugs (Hemiptera) between non-native Eucalyptus benthamii Maiden and Cambage (Camden white gum) and native Pinus taeda L. (loblolly pine) stands on the upper Coastal Plain of South Carolina, U.S.A. We sampled insects emerging from logs of both species placed in both stand types after 1, 2, 6, and 12 months in the field. Beetle and true bug richness and diversity were both significantly lower from eucalypt than from pine wood. Moreover, the two communities were compositionally distinct. Whereas pine supported many species of host-specific phloeoxylophagous beetles, most species collected from eucalypts were mycophagous or predatory taxa capable of utilizing a wide range of hosts. Species richness did not differ between logs placed in eucalypt vs. pine stands but Shannon’s diversity was significantly higher in the eucalypt stands, possibly due to greater sun exposure in the latter. Contrary to a previous study, we found no support for the idea that eucalypt litter reduces the diversity of saproxylic insects. Our findings add to the growing body of evidence that non-native plantations are less favorable to biodiversity than those consisting of native tree species.

enhance the perceived value of this material to saproxylic insect communities at early stages of decay.Because non-native ambrosia beetles typically exhibit a broad host range, it is possible that non-native woods facilitate the proliferation of these species when introduced into new areas.However, no studies, to our knowledge, have explored this possibility.
Non-native plantation forests also have the potential to indirectly affect saproxylic insects by altering the chemistry of the forest floor through inputs of novel leaf litter.Because the litter produced by non-native plants has been shown to reduce the diversity and alter the composition of litter-and soil-dwelling arthropods [12][13][14] , nonnative litter may reduce the suitability of dead wood for saproxylic insects.In Chile, Fierro et al. 5 suggested that the "toxic leaf litter" produced by Eucalyptus contributed to the lower diversity of saproxylic beetles in remnant pine stumps and logs in eucalypt plantations compared to those in non-native pine plantations.However, because the dead wood sampled in the eucalypt and pine plantations likely differed in age (e.g., the eucalypt plantations were about half the age of the pine plantations), it is possible that such differences were unrelated to leaf litter in that study.A more controlled experiment is needed to address this question.
Exhibiting rapid growth rates and tolerating a wide range of soil conditions, commercial eucalypt plantations cover roughly 20 million hectares globally 15 , equivalent to the area of the US state of Nebraska.While Eucalyptus is native to Australia and some neighboring islands, over 95% of eucalypt plantations occur outside of this region, over half of which occurs in Brazil, India and China 15 .As the global area planted in eucalypt continues to grow, there is considerable interest in better understanding the ecological implications of these non-native plantations.Numerous studies have documented the deleterious effects of eucalypt plantations on a variety of native taxa-including herbaceous plants, birds, stream invertebrates, and pollinators [16][17][18] -compared to native forests.Although less studied, saproxylic insects are also thought to benefit little from the woody debris produced by Eucalyptus outside its native range 19 .However, this deserves a closer look given the high diversity of saproxylic insects associated with the genus in Australia 20,21 .
Although fast-growing native pines dominate the timber industry in the southeastern United States, there is some interest in non-native Eucalyptus as an even more productive alternative in places such as Florida where winters are sufficiently mild 22 .However, the ecological implications of such a decision remain largely unknown for this biodiverse region.To better understand the effects of eucalypt plantations on insect diversity, we compared the diversity and composition of saproxylic insects in non-native eucalypt vs. native pine wood at different stages of decomposition on the upper Coastal Plain of South Carolina, U.S.A. Pinus taeda L. was selected for this comparison because it is currently the most widely planted species across the region and is therefore the species most likely to be displaced by eucalypt plantations.We further assessed how stand type (eucalypt vs. pine), and specifically the type of litter beneath woody debris, affected wood colonization by these insects.
We hypothesized that (1) the wood of Eucalyptus would be utilized by fewer insect species, (2) these differences would lessen over time as decomposition progressed, and (3) the wood of both species would be colonized by a greater diversity of insects when placed in pine vs. eucalypt stands.

Methods
This study took place on the Savannah River Site, an 80,000 ha property owned by the US Department of Energy in South Carolina, USA (Fig. 1).The climate is warm temperate with an average high temperature in July of 34.4 °C, an average low temperature in January of 0.6 °C, and an average annual precipitation of 1.3 m.We utilized four locations, each consisting of an experimental Eucalyptus benthamii Maiden and Cambage stand within a matrix of loblolly pine.The eucalypt stands, planted in October 2013, were 0.125 ha in size, with 168 trees in 12 rows of 14 trees, whereas the surrounding loblolly pine stand was planted in January 2013 with a similar spacing and density 23,24 .The four blocks were separated from one another by about 0.1-1.5 km (Fig. 1).At each location, we established one plot within the eucalypt stand and another in the adjacent pine stand, both typically within about 10 m of the boundary between stand types.The ground surface at each eucalypt and pine plot was completely and uniformly covered by a layer of naturally senesced eucalypt and pine litter, respectively.
On 13 April 2018, 12 logs each of eucalypt and pine (cut from living trees felled for this purpose) were placed in each plot, four from each of three diameter classes: small (6.07 ± 0.14 and 6.23 ± 0.15 cm), medium (8.44 ± 0.17 and 8.49 ± 0.15 cm), and large (11.63 ± 0.27 and 11.74 ± 0.31 cm) (values are for eucalypt and pine, respectively).Despite this range in sizes, we acknowledge that this study does not capture the full range of variability in dead wood size or posture.The 12 logs from each species were grouped closely together (but not touching) in three rows by diameter class and the two species groups in each plot were separated by about 1 m (Fig. 1).We returned to collect one log of each diameter class from each species after 1, 2, 6, and 12 months in the field.To prevent the movement of insects between logs from different species or plots during transport, each set of three logs was enclosed within a sealed plastic bag.Each set was then bundled together and placed within an aerated rearing bag to collect emerging insects over a period of 12 months following the methods described by Ulyshen and Hanula 25 .All captured beetles (Coleoptera) and true bugs (Hemiptera) were pinned and identified by MU and ERH to the lowest taxonomic unit possible, typically to species [26][27][28][29] .
Unless otherwise stated, all analyses were conducted in R 30 .We calculated the total richness and Shannon's diversity of beetles and true bugs by block, stand, and month of collection.We also calculated these metrics after pooling across sampling periods.These were the response variables compared among treatments using generalized linear mixed effects models with block (i.e., location) treated as the random term.Initially, stand, wood species, and their interaction were included in the models as fixed effects, but the interaction term was insignificant in all models and was therefore dropped for the final analysis.We used the Poisson and Gaussian distributions for models of richness and diversity, respectively.Finally, to test for compositional differences in communities between wood species, we conducted non-metric multidimensional scaling (NMDS) followed by PERMANOVA in PC-ORD 31 .We ran this analysis for each sampling period separately and also after pooling data across sampling periods.

Collection and use of plant materials
Plants were collected and used on public lands with permission and in accordance with all the relevant guidelines.

Results
A total of 11,440 beetles from at least 95 taxa emerged from all logs over the course of the study (Table 1).Eucalypt logs produced 2674 beetles from 51 taxa while those of pine yielded 8766 beetles from 73 taxa.Overall, about twice as many beetle species on average were collected from pine compared to eucalypt, a significant difference (Table 2, Fig. 2).This difference was consistent across collection periods except for the 1-month sample for which there was no significant difference between wood species (Table 2).Beetle diversity was also significantly higher from pine overall and for the 6-month and 12-month collection periods (Table 2).Additionally, independent of species, beetle diversity was significantly higher from logs placed in eucalypt stands than from those placed in pine stands.This was true overall and for the 2-month and 6-month samples (Table 2).
When analyzing data from each sampling period separately, NMDS and PERMANOVA revealed significant differences in beetle communities between wood species for all sampling periods (Table 3).Only for the 2-month sample did beetle communities differ between stand types (Table 3, Fig. 3).However, there was a significant interaction between stand and wood species for the 12-month sample (Table 3, Fig. 3).When all sampling periods   www.nature.com/scientificreports/were combined, NMDS and PERMANOVA revealed significant differences in beetle communities between both stand and wood species and there was a significant stand × wood interaction (Table 3, Fig. 4).Based on the same combined dataset, NMDS and PERMANOVA also revealed differences in beetle communities between wood species (F 1,56 = 6.93, p < 0.001), among months (F 3,56 = 4.46, p < 0.001), and there was a significant wood × month interaction (F 3,56 = 2.27, p < 0.001) (Fig. 4).Table 1.List of Coleoptera and Hemiptera species that emerged from Eucalyptus and pine after 1, 2, 6, and 12 months in South Carolina, USA.The names of exotic ambrosia beetles are followed by asterisks.Approximate guild designations are as follows: p predator, m mycophage, px phloeoxylophage, i inquiline.Guilds are only assigned to species known or suspected to be saproxylic, otherwise species are marked as "na".saproxylic insects in the U.S., and one of few such studies globally 5 .Although Eucalyptus supports significantly fewer beetle and true bug species than loblolly pine-the most commercially important native timber species in the southern U.S.-we still captured dozens of native species from this novel substrate.However, most of the species collected from Eucalyptus were mycophages and predators (Table 1) which are inherently less host specific than phloeoxylophages, a greater diversity of which emerged from native loblolly pine (21 vs. 5 species).Despite this general pattern, several common native phloeoxylophagous species with broad host ranges were captured from eucalypt wood, including the cerambycid Knulliana cincta (Drury), a species reported previously from Quercus, Carya, Salix, and Ostrya 34 , as well as many bark beetles belonging to the genus Hypothenemus.Not surprisingly given their phylogenetic dissimilarity, eucalypt and pine hosted compositionally distinct insect communities, with several species being found only on eucalypts (e.g., Monarthrum mali (Fitch) and K. cincta) or pine (e.g., Asemum striatum (L.), Astylopsis sexguttata (Say), and Monochamus carolinensis (Olivier)) (Table 1).This was true for every sampling period although, as predicted, the separation between species appeared to decline over time (Fig. 4).

Chrysobothris cribraria
We recorded five non-native ambrosia beetle species from eucalypt logs in this study compared to two species from pine.This suggests that eucalypts may have a facilitative effect on non-native ambrosia beetles in the region.Over 60 species of non-native bark and ambrosia beetles are established in the United States 35,36 , and typically account for a large proportion of ambrosia beetle species and individuals captured in the southeastern U.S. 37,38 .Previous work on the Savannah River Site, where the current study was conducted, found non-native ambrosia beetles species to account for half the species and up to 86% of individuals collected 39,40 .While the ecological implications of many of these species remain poorly understood, these species do not appear to significantly affect the diversity of native bark and ambrosia beetles 41 .
Contrary to Fierro et al. 5 , we found no evidence that eucalypt litter reduces the richness or diversity of saproxylic insects in logs compared to logs placed on native pine litter.In fact, the opposite pattern was observed for insect diversity (Table 2, Fig. 2).It is not clear if this difference is due to differences in leaf litter chemistry or to some variable unrelated to litter that also differed between stands.For example, logs placed in eucalypt stands experienced more sun exposure than those placed in pine stands because eucalypt stands experienced more mortality than pine (due to both windthrow and frost) and maintained less continuous canopies 23 .Moreover, previous studies have shown a positive correlation between sun exposure and beetle diversity in dead wood, possibly because sun exposure enhances microclimatic heterogeneity within and between dead logs 42,43 .Whatever the explanation, our results provide no support for the idea that eucalypt litter reduces the diversity of saproxylic insects associated with dead wood on the forest floor.We suspect the finding reported by Fierro et al. 5 may be more related to differences in wood age between treatments than to the effects of eucalypt litter.

Conclusions
Previous comparisons of saproxylic insect diversity between non-native and native wood species suggest that suitability varies greatly among species and that being non-native to a region does not guarantee that a species will be of little value to saproxylic insects.Indeed, multiple studies have shown that the diversity and composition of insects associated with certain non-native wood species can be comparable to assemblages supported by native wood species [7][8][9][10] .While our findings suggest that many insects species native to the southeastern United States can utilize dead Eucalyptus wood, this taxon supports a much lower diversity of saproxylic insects than loblolly pine, the native timber species most likely to be displaced by Eucalyptus plantations in the region.Such findings are consistent with past research showing that, compared to native forests, non-native tree plantations have a negative effect on numerous taxa [16][17][18] .The current study provides further support for the conclusion that that plantations consisting of native tree species are more beneficial for biodiversity than those consisting of non-native species 3 .

Figure 1 .
Figure 1.Map of study locations on the Savannah River Site in South Carolina.(A,B) Show the placement of logs in Eucalyptus and pine stands, respectively. https://doi.org/10.1038/s41598-024-61193-1

Figure 2 .
Figure 2. Mean ± SE beetle richness (top) and Shannon's diversity (bottom) collected from Eucalyptus and pine wood placed in both stand types (Eucalyptus and pine).Results are shown for collections after 1, 2, 6, and 12 months in the field as well as in total, after summing across collection periods.Asterisks denote significance as follows: ***p < 0.001, **p < 0.01, *p < 0.05.

Figure 4 .
Figure 4. NMDS ordination for beetle communities emerging from logs for all months combined.Samples are grouped by stand type and wood species (left panel) and by wood species and month (right panel).

Table 3 .
Results of two-way PERMANOVA showing effects of stand type, wood species, and their interaction on the composition of beetles emerging from dead logs.